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| | PhosphoDetect™ Anti-Chk1 (pSer317) Rabbit pAb |
|  | Cat. No. DR1025 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser317 phosphorylation site of human Chk1 | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: Lysates from COS cells treated with UV | | Comments: Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells. | | Ref.: Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129. Shieh, S.Y., et al. 2000. Genes Dev. 14, 289. Martinho, R.G., et al. 1998. EMBO J. 17, 7239. Zeng, Y., et al. 1998. Nature 395, 507. |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | human, monkey, mouse, rat | IB, IC, PS
See Key |
Detection of human and monkey Chk1, phosphorylated at Ser317, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer317) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence.
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