 |
 | 2 to 8°C |  | Blue ice |  | Highly Toxic | | Note: Store and Ship conditions may differ. | | See Key |
|
|
|
|
| | Anti-PARC/H7-AP1 (900-950) Rabbit pAb |
|  | Cat. No. DR1028 |
|
Anti-p53 Associated Parkin-Like Cytoplasmic Protein
Anti-UbcH7 Associated Protein 1 | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids 900-950 of human PARC | | Form: Liquid | | Formulation: In Tris-citrate/phosphate buffer, pH 7.0-8.0. | | Preservative: ≤0.1% sodium azide | | Positive Control: U2OS cells | | Comments: Recognizes the ~270 kDa PARC/H7-AP1 protein in U20S cells. | | Ref.: Nikolaev, A.Y., et al. Cell 112, 29. |
|
 | Related information for this product is available: | |
| |
EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
Lanes 1, 2: Detection of human PARC/H7-AP1 by immunoblotting (Lanes 1 and 2). Samples: Whole cell lysate (40 µg) from untreated U2OS cells (Lane 1) or U2OS cells that stably express a short hairpin RNA (shPARC) against PARC/H7-AP1 (Lane 2). Primary antibody: Anti-PARC/H7-AP1 (900-950) Rabbit pAb (Cat. No. DR1028) (1:2500). Detection: chemiluminescence. Lanes 3, 4: Detection of human PARC/H7-AP1 by immunoprecipitation followed by immunoblotting. Samples: Whole cell lysate (500 µg) from untreated U2OS cells. Antibodies for IP: Normal rabbit IgG (2 µg/mg lysate) (Lane 3) and Anti-PARC/H7-AP1 (Cat. No. DR1028) (2 µg/mg lysate) (Lane 4). Immunoblotting conditions: same as Lanes 1 and 2.
|
|
|
