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| | Anti-WRN (400-450) Rabbit pAb |
|  | Cat. No. DR1032 |
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Anti-HGNC:12791
Anti-RECQ3
Anti-RECQL2
Anti-Werner Syndrome Helicase
Anti-Werner Syndrome Protein | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids encoded within residues 400-450 of human WRN (using the numbering in SwissProt entry Q14191 | | Form: Liquid | | Formulation: In Tris-citrate/phosphate buffer, pH 7.0-8.0. | | Preservative: ≤0.1% sodium azide | | Positive Control: HEK293T cells | | Comments: Recognizes the ~160 kDa WRN protein in HEK293T cells. | | Ref.: Comai, L. and Li, B. 2004. Mech. Ageing Dev. 125, 521. Kipling, D., et al. 2004. Science 305, 1426. Bachrati, C.Z., et al. 2003. Biochem. J. 374, 577. Marciniak, R.A., et al. 1998. J. Biol. Chem. 95, 6887. |
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IB (far left lane; lane 1): Detection of human WRN by immunoblotting. Sample: Whole cell lysate (100 µg) from HEK293T cells. Primary antibody: Anti-WRN (400-450) Rabbit pAb (Cat. No. DR1032) (0.1 µg/ml) pre-incubated without peptide. Detection: chemiluminescence. IP/IB (remaining lanes; lanes 2-6): Detection of human WRN by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (one 10 cm plate) from HEK293T cells. Antibody for IP: Anti-WRN (400-450) Rabbit pAb (Cat. No. DR1032) (5 µg/10 cm plate) (lanes 3-6) and IgG IP control (lane 2). Immunoblotting conditions: Primary antibody: Anti-WRN (400-450) Rabbit pAb (Cat. No. DR1032) (0.1 µg/ml) pre-incubated without peptide (lanes 2,4,6) or with immunogen peptide (lane 3,5). Detection: chemiluminescence.
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