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| | Anti-Plk1 Mouse mAb (35-206) |
|  | Cat. No. DR1037 |
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Anti-Polo-Like Kinase | Host: Mouse | | Isotype: IgG2b | | Immunogen: full-length, recombinant, human Plk1 | | Form: Liquid | | Formulation: In PBS. | | Preservative: ≤0.1% sodium azide | | Positive Control: Nocodazole-treated HeLa cells | | Comments: Recognizes the ~62 kDa Plk1 protein in HeLa cells. | | Ref.: Takai, N., et al. 2005. Oncogene 24, 287. Toyoshima-Morimoto, F., et al. 2002. EMBO Rep. 4, 341. Smits, V.A., et al. 2000. Nat. Cell Biol. 2, 672. Glover, D.M., et al. 1998. Genes Devel. 12, 3777. |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| 35-206 | human, mouse, rat | IB, IP
See Key |
Detection of human Plk1 by immunoblotting. Sample: Whole cell lysate (50 µg) from HeLa cells treated with nocodazole (100 ng/ml). Primary antibody: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (1 µg/ml). Detection: chemiluminescence.
Detection of human Plk1 by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (446 µg) from HeLa cells treated with nocodazole (100 ng/ml). Antibody for IP: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (5 µg). Conditions for immunoblotting: same as above.
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