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| | PhosphoDetect™ Anti-IRS-1 (pSer616) Rabbit pAb |
|  | Cat. No. GF1001 |
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Anti-Insulin Receptor Substrate-1, Phospho-Specific (Ser616) | Note: 1T = 1 test. Sufficient for 10 immunoblots. | | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide surrounding the Ser616 phosphorylation site of human IRS-1 | | Form: Liquid | | Formulation: In phosphate buffer, 1 mg/ml BSA, pH 7.4. | | Preservative: ≤0.1% sodium azide | | Positive Control: Insulin treated 3T3-L1 adipocytes or IRS-1 transfected CHO-T cells | | Comments: Recognizes the ~165-180 kDa IRS-1 protein phosphorylated at Ser616 in IRS-1 transfected CHO-T cells. | | Ref.: Aguirre, V., et al. 2002. J. Biol. Chem. 277, 1531. Hers, I., et al. 2002. Biochem. J. 368, 875. Reiss, K., et al. 2001. Oncogene 20, 490. |
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Detection of human IRS-1, phosphorylated at Ser616, by immunoblotting. Samples: Whole cell lysate from CHO-T cells mock transfected and treated with TPA (lanes 1,4), transiently transfected with wild-type human IRS-1 (lanes 2, 5), or transiently transfected with mutant human IRS-1 (S616A lanes 3, 6). Primary antibodies: PhosphoDetect™ Anti-IRS-1 (pSer616) Rabbit pAb (Cat. No. GF1001) (lanes 1-3) or anti-IRS-1 pan antibody (lanes 4-6) (0.50 µg/ml). Detection: chemiluminescence.
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