| Form: 50 Tests | | Detection Method: Microscopy | | Assay Time: ~2.5 hours plus labeling time | | Sample Type: Paraffin-embedded tissues or fixed cells | | Positive Control: Any cell of tissue sample labeled with BrdU | | Kit Contents: Trypsin concentrate, trypsin diluent, denaturing solution, blocking solution, biotinylated mouse anti-BrdU, streptavidin-peroxidase, substrate buffer reaction mix, DAB concentrate, hematoxylin, mounting media, control slides (1 stained and 4 unstained), and a user protocol. | | Comments: Useful for the detection of proliferating cells within fixed tissue samples or cell populations. Requires metabolic incorporation of BrdU (Cat. No. 203806) into sample prior to paraffin-embedding. | | Ref.: Zhang, Q. and Paria, B.C. 2006. Endocrinology 147, 2215. Fukuda, K ., et al. 1990. Anal. Quant. Cytol. Histol. 12, 135. Cattoretti, G. et al. 1993. J. Pathol. 171, 83. Schutte, B., et al. 1987. J. Histochem. & Cytochem. 35,1343. Gonchoroff , N.J., et al. 1986. J. Immunol. Meth. 93, 97. Ellwart, E. and Dormer, P. 1985. Cytometry 6, 513. Gratzner, H.G., et al. 1975. Expl. Cell Res. 95, 88. | | R: 25-36/37/38-42/43-61; S: 24-26-36/37/39-45 |
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