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 | 2 to 8°C |  | Blue ice |  | Highly Toxic | | Note: Store and Ship conditions may differ. | | See Key |
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| | Anti-MTA3 (400-450) Rabbit pAb |
|  | Cat. No. IM1012 |
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Anti-Metastasis-associated gene 3 | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids within residues 400-450 of human MTA3 | | Form: Liquid | | Formulation: In Tris-citrate/phosphate buffer, pH 7.0-8.0. | | Preservative: ≤0.1% sodium azide | | Positive Control: MCF7 cells | | Comments: Recognizes the ~62 kDa MTA3 protein in MCF-7 cells. | | Ref.: Fujita, N., et. al. 2003. Cell 113, 207. Kumar R. 2003. Cell 113, 142. Simpson, A, et.al. 2001. Gene 273, 29. |
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Lane 1: Detection of human MTA3 by immunoblotting. Samples: Nuclear extract (25 µg) from MCF-7 cells. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:1000). Detection: chemiluminescence. Lanes 2 and 3: Detection of human MTA3 by immunoprecipitation and immunoblotting. Samples: Whole cell lysate from MCF-7 cells (500 µg) (lane 2). Antibody for IP: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:100) and immunoprecipitation control (lane 3): nonspecific rabbit IgG (1:100). Immunoblotting conditions: same as lane 1. Detection: chemiluminescence.
Detection of human MTA3 by immunocytochemistry. Sample: Sk-Mel-28 cells fixed for 5 min with ice cold methanol and blocked with normal goat serum. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:300). Detection: fluorescence.
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