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| | PhosphoDetect™ Anti-Neurofilament H Mouse mAb (SMI-31) |
|  | Cat. No. NE1022 |
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| Host: Mouse | | Isotype: IgG1 | | Immunogen: homogenized, hypothalami from Fischer 344 rat brain | | Form: Liquid | | Formulation: Undiluted ascites. | | Preservative: ≤0.1% sodium azide | | Positive Control: Rat brain or central nervous system cytoskeletal preparations | | Comments: Recognizes the ~180-200 kDa phosphorylated neurofilament H protein in rat central nervous system (CNS) cytoskeletal preparations. Also weakly recognizes phosphorylated NF-M protein. | | Ref.: Raina, A.K., et al. 1999. Neuroreport 10, 1355. Yang, C.C., et al. 1998. Brain 121, 1089. Giasson, B.I and Mushynski, W.E. 1996. J. Biol. Chem. 271, 30404. Mirabella, M., et al. 1996. J. Neuropath. Exp. Neurol. 55, 774. Xiao, J. and Monteiro, M.J. 1994. J. Neurosci. 14, 1820. |
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| SMI-31 | chicken, mammalian, Xenopus | ELISA, FS, IB, IC, PS
See Key |
Detection of phosphorylated rat neurofilament H by staining frozen sections. Sample: Rat brain. Primary antibody: PhosphoDetect™ Anti-Neurofilament-H Mouse mAb (SMI-31) (Cat. No. NE1022) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
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