Host: Mouse

Isotype: IgG2a

Immunogen: partially purified murine p53

Epitope: within amino acids 371-380 of human p53

Form: Liquid

Formulation: In 50 mM sodium phosphate buffer, 50% glycerol.

Preservative: None

Positive Control: Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)

Negative Control: Normal skin tissue or SK-OV-3 cells

Comments: Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated or acetylated p53.

Ref.: El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Legros, Y., et al. 1994. Oncogene 9, 2071. Barak, Y., et al. 1993. EMBO J. 12, 461. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Kastan, M.B., et al. 1992. Cell 71, 587. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51 6304. Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709. Harlow, E., et al. 1981. J. Virol. 39 861.