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  Anti-p21WAF1 (Ab-1) Mouse mAb (EA10)
Cat. No. OP64  
All Categories » Calbiochem » Antibodies » Primary Antibodies » Cell Cycle Control Proteins

Anti-CIP1
Anti-p21
Anti-SD11
Anti-WAF

Host: Mouse
Isotype: IgG1
Immunogen: full-length, recombinant, human p21WAF1
Epitope: within amino acids 58-77 of human p21WAF1
Form: Liquid
Formulation: In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Preservative: ≤0.1% sodium azide
Positive Control: Any cell line expressing wild-type p53 (e.g. Hs27 or U2OS treated with DNA-damaging agents) or skin or colon tissue
Negative Control: Unstimulated cells, unstimulated skin tissue, or SK-OV-3 cells
Comments: Recognizes the ~21 kDa p21WAF1 protein in skin and colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
Ref.: Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493. Chen, Y.Q., et al. 1995. Int. J. Oncology 7, 889. Deng, C., et al. 1995. Cell 82, 675. El-Deiry, W.S., et al. 1995. Cancer Res. 55, 2910. Waldman, T., et al. 1995. Cancer Res. 55, 5187. Elbendary, A., et al.1994. Cell Growth Diff. 5, 1301. El-Deiry, W.S., et al. 1994 Cancer Res. 54, 1169. Li, R., et al. 1994. Nature 371, 534. Michieli, P., et al. 1994. Cancer Res. 54, 3391. Noda, A., et al.1994. Exp. Cell Res. 211, 90. El-Deiry, W.S., et al.1993. Cell 75, 817. Gu, Y., et al. 1993. Nature 366, 707. Harper, J.W., et al.1993. Cell 75, 805. Xiong, Y., et al.1993. Genes Devel. 7, 1572. Xiong, Y., et al.1993. Nature 366, 701. Xiong, Y., et al.1992. Cell 71, 505.

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20 µgY$89.00
100 µgY$268.00


CloneSpecies ReactivityApplication
EA10 human 
not mouse, not rat 
FC, FS, IB, IF, IP, PS 
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Detection of p21 WAF1 by immunoblotting: Sample: Whole cell lysate (56 µg) from MCF-7 cells. Primary antibody: Anti-p21WAF1 (Ab-1) Mouse mAb (EA10) (Cat. No. OP64) (3 µg/ml). Secondary antibody: Anti-Mouse IgG (Goat) Peroxidase Conjugate. Detection: chemiluminescence.

Related Products:
OP64: Anti-p21WAF1 (Ab-1) Mouse mAb (EA10)
OP64F: Anti-p21WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate

Related Literature:

DNA Repair Pathway Poster

Antibodies for Cancer Research Product Guide

p53 And Related Products

Material Safety Data Sheets:
OP64: Anti-p21WAF1 (Ab-1) Mouse mAb (EA10) - English
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Related Categories:
All Categories » Calbiochem » Antibodies » Primary Antibodies » Cell Cycle Control Proteins