Host: Sheep

Isotype: IgG

Immunogen: purified, human breast carcinoma pro-MMP-1

Form: Liquid

Formulation: In phosphate buffer, 0.2% BSA.

Preservative: ≤0.1% sodium azide

Positive Control: Conditioned medium from PMA-treated HT-1080 cells

Comments: Recognizes the ~55 kDa latent and the ~43 kDa active forms of MMP-1 in PMA-treated HT1080 cells. In some cell lines, (i.e. stimulated, connective tissue cells) a minor glycosylated form of MMP-1 may be seen as a doublet of 55-57 kDa.

Ref.: Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861. Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434. Woessner, J.F. 1991. FASEB J. 5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In: Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.