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| | Anti-p53 (Ab-7) (Pantropic) Sheep pAb |
|  | Cat. No. PC35 |
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| Host: Sheep | | Isotype: IgG | | Immunogen: recombinant, human p53 protein | | Form: Liquid | | Formulation: 200 µl diluted (1:4) anti-p53 sheep serum, 500 µl undiluted normal sheep serum, and 100 µl biotinylated rabbit anti-sheep IgG. | | Preservative: ≤0.1% sodium azide (Anti-p53 and Anti-Sheep IgG only) | | Positive Control: A431 or SVT2 cells or breast carcinoma tissue | | Kit Contents: Anti-p53 antisera, normal sheep serum, and biotinylated rabbit anti-sheep IgG | | Comments: Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 and SVT2 cells and breast carcinoma tissue. | | Ref.: El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51, 6304. |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | human, mouse, rat | FS, IB, IF, IP, PS
See Key |
Detection of mouse p53 by immunoblotting. Sample: Whole cell lysate (10 mg) from SVT2 cells. Primary antibody: Anti-p53 (Ab-7) (Pantropic) Sheep pAb (Cat. No. PC35) (1:2500) (lane 1) or normal sheep serum (lane 2). Detection: chemiluminescence.
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