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| | PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb |
|  | Cat. No. PC386T |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser15 phosphorylation site of human p53, conjugated to KLH | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: ≤0.1% sodium azide | | Positive Control: PC-12, HCT116, or HeLa cells treated with DNA damaging agents | | Comments: Recognizes the ~53 kDa p53 protein phosphorylated at Ser15 in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize non-phosphorylated p53. | | Ref.: Tibbetts, R.S., et al. 1999. Genes Dev. 13, 152. Levine, A.J. 1997. Cell 88, 323. Milczarek, G.J., et al. 1997. Life Sci. 60, 1. Shieh, S.Y., et al. 1997. Cell 91, 325. Meek, D.W. 1994. Semin Cancer Biol. 5, 203. |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | human, mouse, rat | FFS, IB, IC, IP, PS
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb | PC386 | 50 µl | Y | N/A |
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Detection of human p53, phophorylated on Ser15, by immunoblotting. Sample: Whole cell lysates from untreated HCT116 cells (Lane A) and HCT116 cells treated with ionizing radiation (Lane B). Primary antibody: PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb (Cat. No. PC386) (1:1000). Detection: chemiluminescence.
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