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| | Anti-Vanilloid Receptor Like Protein-1 (744-761) Rabbit pAb | |  | Cat. No. PC421 | |
Anti-TRPV2 | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide (KNSASEEDHLPLQVLQSP) corresponding to amino acids 744-761 of rat vanilloid receptor like protein 1, conjugated to KLH | | Form: Liquid | | Formulation: 100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide (immunogen) (100 µg size only). | | Preservative: ≤0.1% sodium azide (antibody only) | | Positive Control: Rat or mouse spinal cord extract | | Comments: Recognizes the ~98 kDa (doublet) vanilloid receptor like protein-1 in rat spinal cord extract. Supplied with a control peptide (100 µg size only). | | Ref.: Strokes, A.L., et al., 2004. J. Exp. Med. 200, 137. Caterina, M.J., et al., 1999. Nature 398, 436. Gau, A., et al., 1999. Eur. J. Neurosci. 11, 946. Michael, G.J. and Priestley, J.V., 1999. J. Neurosci. 19, 1844. Caterina, M.J., et al., 1997. Nature 389, 816. Szallasi, A., 1994. Gen. Pharmacol. 25, 223. Beven, S., and Szolcsanyi, J., 1990. Trends Pharmacol. Sci. 11, 330. Fields, H.L., 1987. Pain (New York: McGraw-Hill), 1987. | |
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 Detection of rat vanilloid-receptor-like protein 1 by immunoblotting. Sample: Whole tissue extract from rat spinal cord pre-incubated without peptide (lane 1) or with control peptide (25 µg/ml, lane 2). Primary antibody: Anti-Vanilloid Receptor Like Protein-1 (744-761) Rabbit pAb (Cat. No. PC421) (2 µg/ml). Detection: chemiluminescence.
 Detection of mouse vanilloid-receptor-like protein 1 by staining frozen sections. Sample: Mouse spinal cord dorsal horn fixed in 4% paraformaldehyde. Primary antibody: Anti-Vanilloid Receptor Like Protein-1 (744-761) Rabbit pAb (Cat. No. PC421) (1 µg/ml). Staining was completely abolished by preincubating the purified antibody with control peptide at 10-6 M (data not shown).
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