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| | PhosphoDetect™ Anti-Bad (pSer136) (Ab-2) Rabbit pAb |
|  | Cat. No. PC519 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser136 phosphorylation site of mouse Bad | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol. | | Preservative: None | | Positive Control: Bad control proteins | | Comments: Recognize the ~23 kDa Bad protein phosphorylated at Ser136 in TPA treated Bad-transfected cells. | | Ref.: Datta, S.R., et al. 1997. Cell 91, 231. Mizushima, S. and Nagata, S. 1997. Science 18, 5322. Peso, L., et al. 1997. Science 278, 687. Zha, J., et al. 1996. Cell 87, 619. Yang, E., et al. 1995. Cell 80, 285. |
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Detection of Bad, phosphorylated at Ser136, by immunoblotting. Samples: Whole cell lysate from HEK293 cells transfected with wild-type Bad or Bad mutants, S112A, S136A, S112A/S136A, left untreated, treated with TPA or forskolin as indicated. Primary antibody: PhosphoDetect™ Anti-Bad (pSer136) (Ab-2) Rabbit pAb (Cat. No. PC519) (1:500). Detection: chemiluminescence.
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