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| | Anti-Active Caspase-7 Rabbit pAb |
|  | Cat. No. PC520 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to the amino acids surrounding the human caspase-7 cleavage site | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: Cytochrome c-treated Jurkat cells | | Negative Control: Untreated Jurkat cells | | Comments: Recognizes the ~20 kDa active form of caspase-7 in treated Jurkat or 293 cells. Does not cross-react with the latent form of caspase-7. | | Ref.: Nunez, G., et al. 1998. Oncogene. 17, 3237. Chandler, J.M., et al. 1997. J. Biol. Chem. 273, 10815. Cohen, G.M., 1997. Biochem J. 326, 1. MacFarlane, M., et al. 1997. J. Chem. Biol. 137, 469. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907. Duan, H., et al. 1996. J. Biol. Chem. 271, 1825. Lippke, J.A, et al. 1996. J. Biochem. 271, 1825-1828. Fernandes-Alnemri, T., et al. 1995. Cancer Res. 55, 6045. |
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EMD Chemicals Inc. GBP list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | human, mouse, rat | IB, IF, IP
See Key |
Detection of human active caspase-7 by immunoblotting. Sample: Whole cell lysate from Jurkat and HEK293 cells left untreated (-), treated with cytochrome c (250 µg/ml, 1h, cytc), or treated with etoposide (25 µM, 5h, eto). Primary antibody: Anti-Active Caspase-7 Rabbit pAb (Cat. No. PC520) (1:1000). Detection: chemiluminescence.
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