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| | Anti-ADAM10 (Ab-1) (732-748) Rabbit pAb |
|  | Cat. No. PC528 |
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Anti-Kuz | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide (PQRQRPRESYQMGHMRR) corresponding to amino acids 732-748 of human ADAM10 | | Form: Liquid | | Formulation: In PBS. | | Preservative: ≤0.1% sodium azide | | Positive Control: Jurkat cell lysate | | Comments: Recognizes the ~85 kDa ADAM10 precursor, a faint 60 kDa band corresponding to processed mature protein, and a 45 kDa degradation product in Jurkat cells. | | Ref.: Qi, H., et al. 1999. Science 283, 91. Pan, D. and Kuzbanian, G.M. 1997. Cell 90, 271. Rosendahl, M.S., et al. 1997. J. Biol. Chem. 274, 24588. Howard, L., et al. 1996. Biochem. J. 317, 45. |
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Detection of human ADAM10 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ADAM10 (Ab-1) Rabbit pAb (Cat. No. PC528) (1:1000) pre-incubated without (lane 1) or with (lane 2) blocking peptide. Detection: chemiluminescence
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