Detection of human Bad, phosphorylated at Ser 136, by immunoblotting. Samples: Whole cell lysate from HEK293 cells expressing wild-type BAD (lanes 1, 3) or mutant BAD S136A (lane 2) and activated by Akt (lanes 1-3). Membranes. Primary antibody: Anti-Bad, Phospho-Specific (Ser136) (Cat. No. PC637), pre-incubated with non-phosphopeptide corresponding to the immunogen (lane 1) with immunogen or phosphopeptide immunogen (lane 2), (0.5 µg/ml) and Anti-Bad (total). Detection: chemiluminescence.
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