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| | PhosphoDetect™ Anti-elF-2a (Ab-1) (pSer52) Rabbit pAb |
|  | Cat. No. PC638 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser51 phosphorylation site of Drosophila elF-2a (human phosphorylation site is Ser52) | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3 (IgG, protease-free). | | Preservative: ≤0.1% sodium azide | | Positive Control: HeLa, MCF7, CEM or LIF-treated 3T3-L1 cells | | Comments: Recognizes the ~36 kDa elF-2a protein phosphorylated at Ser52 in HeLa, MCF-7, CEM, and LIF-treated 3T3-L1 cells. | | Ref.: Patel, C.V., et al. 2000. J. Biol. Chem. 275, 37993. Lu, J., et al. 1999. J. Biol. Chem. 274, 32198. Satoh, S., et al. 1999. Biochem. J. 342, 65. |
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Detection of human elF-2a by immunoblotting. Sample: Whole cell lysate from 3T3-L1 adipocytes treated with LIF. Primary antibody: PhosphoDetect™ Anti-elF-2a (Ab-1) (pSer52) Rabbit pAb (Cat. No. PC638) (1:1000) pre-incubated without peptide (lane 1), with non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3), or the phosphopeptide immunogen (lane 4). Detection: chemiluminescence.
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