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| | Anti-Bcl-x (Ab-1) (201-216) Rabbit pAb |
|  | Cat. No. PC67 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide (PWIQENGGWDTFVELY) corresponding to amino acids 201-216 of human Bcl-x | | Form: Liquid | | Formulation: In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5. | | Preservative: ≤0.1% sodium azide | | Positive Control: Doxorybicin-treated MCF-7 cells (see comments) or intestinal tissue | | Negative Control: Brain tissue | | Comments: Recognizes the ~28 kDa Bcl-x protein in doxorubicin-treated MCF-7 cells. | | Ref.: Datta R., et al. 1995. Cell Growth Differ 6, 363. Fang W., et al. 1995. J. Immunol. 155, 66. Gonzalez-Garcia M., et al. 1994. Development 120, 3033. Krajewski S., et al. 1994. Cancer Res 54, 5501. Boise L.H., et al. 1993. Cell 74, 597. |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | human, mouse, opossum, rat | FS, IB, PS
See Key |
Detection of human Bcl-x by immunoblotting. Sample: Whole cell lysate from MCF7 cells treated with doxorubicin (Cat. No. 324380) (200 ng/ml for 72 h). Primary antibody: Anti-Bcl-x (Ab-1) (201-216) Rabbit pAb (Cat. No. PC67) (lane 1) (2.5 µg/ml) and normal rabbit IgG, negative control (lane 2) (2.5 µg/ml). Detection: chemiluminescence.
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