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 | 2 to 8°C |  | Blue ice |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | Annexin V-FITC Apoptosis Detection Kit |
|  | Cat. No. PF032 |
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| Format: Flow cytometry or fluorescence microscopy | | Form: 100 Tests | | Detection Method: Fluorometric | | Species Reactivity: a broad range of species | | Assay Time: 15 - 30 min | | Sample Type: Intact cells | | Positive Control: Apoptotic Jurkat cells (dexamethasone or anti-Fas treated) | | Kit Contents: Annexin V-FITC, 5X Binding Buffer, Propidium Iodide, RAPID™ Media Binding Reagent, and a user protocol. | | Comments: Optimized for flow cytometry and fluorescence microscopy applications. A RAPID™ protocol has been developed for Annexin V-FITC binding directly in tissue culture media. This obviates the need for tedious centrifugation and wash steps, that increase the occurrence of mechanical membrane disruption. In addition, since apoptosis is a dynamic process that is ongoing once cells are removed from culture conditions and continues throughout experimental processing, the RAPID™ protocol is recommended for the detection of cells in early apoptosis. | | Ref.: Frey, T. 1997. Cytometry 28, 253. Darzynkiewicz. Z., et al. 1997. Cytometry 27, 1. Boersma, A.W.M., et al. 1996. Cytometry 24, 123. Wyllie, A. H. 1993. Br. J. Cancer 67, 205. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. Fadok, V.A., et al. 1992. J. Immunology 148, 2207. Kerr, J.F.R., et al. 1972. Cancer 26, 239. | | R: 36; S: 22-24 | | Sold under license of U.S. Patent 5,834,196. |
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FITC Log Plot. Viable cells do not bind Annexin V-FITC or Propidium Idide (PI) as reflected in the lower left-hand quadrant of the dot plot. Early apoptotic cells with exposed PS but intact cell membranes bind Annexin V-FITC but exclude PI. Fluorescence from this population is reported in thelower right-hand quadrant. Necrotic or apoptotic cells in terminal stages will be both Annexin V-FITC and PI positive and will be reported in the upper right-hand quadrant. A small percentage of normal cell death should be expected in routine cultures of untreated cells.
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| - Bysani Chandrasekar, et al. (2005) The pro-atherogenic cytokine interleukin-18 induces CXCL16 expression in rat aortic smooth muscle cells via MyD88, interleukin-1 receptor-associated kinase, tumor necrosis factor receptor-associated factor 6, c-Src, phosphatidylinositol 3-kinase, Akt, c-J. Journal of Biological Chemistry 280, 26263-26277.
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