Calbiochem: PF036: Annexin V-Biotin Apoptosis Detection Kit

Tech Resources
	User Protocol
	MSDS - English
	Other Languages
	Certificate of Analysis

Store
	2 to 8�C
Ship
	Blue ice
	Multiple Toxicity Values, refer to MSDS
Note: Store and Ship conditions may differ.
See Key

��	Annexin V-Biotin Apoptosis Detection Kit

Cat. No. PF036��

All Categories � Calbiochem � Kits and Tools � Apoptosis Activity and Detection Kits � Other

Format: Flow cytometry or fluorescence microscopy
Form: 100 Tests
Detection Method: Fluorescence
Species Reactivity: a broad range of species
Assay Time: 15 - 30 min
Sample Type: Intact cells
Positive Control: Apoptotic Jurkat cells (dexamethasone or anti-Fas treated)
Kit Contents: Annexin V-biotin, 5X Binding Buffer, Propidium Iodide, RAPID™ Media Binding Reagent, and a user protocol.
Comments: Novel RAPID™ protocol and easy sample preparation. Optimized for flow cytometry and fluorescence microscopy applications. Useful for detection of membrane changes during apoptosis. Also useful in conjugation with GFP or other fluorophores.
User provides appropriately labeled streptavidin.
Ref.: Darzynkiewicz, Z., et al. 1997. Cytometry 27, 1. Frey, T. 1997. Cytometry 28, 253. Boersma, A.W.M., et al. 1996. Cytometry 24, 123. White, E. 1996. Genes and Devel. 10, 1. Martin, S.J., et al. 1995. J. Exp. Med. 182, 1545. Koopman, G., et al. 1994. Blood 84, 1415. Wyllie, A.H. 1993. Br. J. Cancer 67, 205. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. Fadok, V.A., et al. 1992. J. Immunology 148, 2207. Schmid, I., et al. 1992. Cytometry 13, 204. Kerr, J.F.R., et al. 1972. Cancer 26, 239.
Sold under license of U.S. Patent 5,834,196.

Need additional information about this product? Email our Technical Service department at: technical@calbiochem.com

� Related information for this product is available:
Additional information is available from the "Tech Resources"
box in the upper left panel of this page.

EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

Sales Office Contact Details

Size
In Stock
Select Country Above
For Pricing Information

1 ea Y N/A

FITC Plot A. RAPID™ Annexin V-Biotin binding is identical to results with the conventional protocol. Jurkat cells incubated with 0.5 mg/ml Actinomycin D (Cat. No. 114666) for either 5 (middle) or 19 (bottom) hours. Untreated Jurkats are shown in the top panel of each section. Annexin V-Biotin was then bound in either binding buffer (using the conventional protocol) or directly in media (RAPID™ protocol). SA-FITC was used to detect bound Annexin V-Biotin.

FITC Plot B. See FITC Plot A.

Material Safety Data Sheets:
PF036: Annexin V-Biotin Apoptosis Detection Kit - English
Bulgarian
Danish
Dutch
Finnish
French
German
Greek
Hungarian
Italian
Korean
Norwegian
Polish
Portuguese
Spanish
Swedish

Related Categories:
All Categories � Calbiochem � Kits and Tools � Apoptosis Activity and Detection Kits � Other

Change Country | Contact Us | Privacy Policy | Terms and Conditions

©2008 Calbiochem, Novabiochem, & Novagen

are brands of EMD Chemicals Inc., an Affiliate of Merck KGaA, Darmstadt, Germany.