Lyophilized. Lyophilized from 100 mM NaCl, 50 mM HEPES, pH 7.3. AVOID FREEZE/THAW CYCLES. Recombinant, human pro-MMP-3 purified from cell culture supernatant. May be used as a positive control or standard for zymographic analysis, or substrate assay. Requires activation for immunoblotting, prior to use. M.W. 57000/58000. Activity: The activity of proenzyme MMP 3 was measured by substrate cleavage assay using 0.5 mM thiopeptiolide (Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-Oet) as a substrate. The activity was also assessed by degradation of a peptide substrate (DNP-PYAYWMR) using activated MMP-3 as measured by HPLC.. Purity: ≥95% by SDS-PAGE. EC 3.4.24.17.

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