HB-EGF

Solid. Lyophilized from a sterile-filtered PBS solution, 50 µg of BSA/µg of cytokine, pH 7.4. AVOID FREEZE/THAW CYCLES. Recombinant, human heparin binding epidermal growth factor expressed in S. frugiperda insect cells. The mature HB-EGF is generated by the removal of the 62 amino acid (~9.5 kDa) signal. Protein is heterogeneously O-glycosylated and migrates as an ~12 kDa protein in SDS-PAGE. Titrate the protein for optimal results in individual systems. Biological activity: EC₅₀ of 2-5 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in the EGF-responsive fibroblast cell line, Balb/3T3. Purity: ≥97% by SDS-PAGE. Contaminants: Endotoxins: ≤1 EU per µg cytokine.

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