Lyophilized. Lyophilized from PBS. AVOID FREEZE/THAW CYCLES. Recombinant, human Fas ligand (amino acids 103-281) fused to a 26-amino acid linker and FLAG®-tag at the N-terminus and expressed in HEK293 cells. Does not require enhancer for activity. Suitable for cytotoxicity assays. M.W. 32,000 - 35,000. Biological activity: ≥1 ng/ml kills Fas-sensitive cells. Purity: ≥95% by SDS-PAGE. Ref.: Ashkenazi, A., and Dixit, V.M. 1998. Science 281, 1305. Nagata, S., 1997 Cell 88, 355. Hahne, M., et al. 1996. Science 274, 1363. Bellgrau, D., et al. 1995. Nature 377, 630. Lowin, B., et al. 1994. Nature 370, 650. Russell, J.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 4409. |
