Liquid. In 200 mM NaCl, 50 mM Tris-HCI, 5 mM CaCl2, 1 µM ZnCl2, 0.05% BRIJ®-35 Detergent, pH 7.0. AVOID FREEZE/THAW CYCLES. Native MMP-2 from mouse calvariae. Pro-MMP-2 (progelatinase A) is cleaved in vivo by MT2-MMP to yield a 67 kDa intermediate and the 62 kDa active enzyme. Activity: ≥500 mU/mg after activation with APMA.. Specific activity: ≥400 mU/mg protein. One unit is defined as the amount of enzyme that can hydrolyze 1 µmol of the substrate N-(2,4)-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0. Purity: ≥90% by SDS-PAGE. Ref.: Knight, C.G., et al. 1992. FEBS Lett. 296, 215. |
