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| | Bcl-2, C-Terminal Truncated, His•Tag®, Human, Recombinant, E. coli | |  | Cat. No. PF126 | |
Liquid. In 100 mM KCl, 10 mM HEPES, 20% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His•Tag® sequence, and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members. Biological activity: EC50 = 100-300 nM based on its ability to inhibit 54 nM caspase-8-cleaved human Bid-mediated release of cytochrome c from isolated mouse liver mitochondria. Purity: ≥95% by SDS-PAGE. |
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