Second Mitochondria Derived Activator of Caspase/DIABLO Liquid. In 2 µm sterile-filtered 100 mM KCl, 20 mM HEPES, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human Smac/DIABLO (amino acids 56-239) fused to a His•Tag® sequence at the C-terminus and expressed in E. coli. Smac/DIABLO is released along with cytochrome c during apoptosis and promotes caspase activation through the neutralization of a family of apoptosis inhibitory proteins. Biological activity: IC50: ~150-750 nM for reversal of the inhibition of XIAP/BIR3 (200 nM) on Z-DEVD-AFC (Cat. No. 264150) cleavage in activated cell extracts; IC50: ~500-1500 nM for reversal of the inhibition of XIAP (500 nM) on Z-DEVD-AFC (Cat. No. 264150) cleavage in activated cell extracts.. Purity: ≥95% by SDS-PAGE. Ref.: Ekert, P., et al. 2001. J. Cell Biol. 152, 483. Chai, J., et al. 2000. Nature 406, 855. Du, C., et al. 2000. Cell 102, 33. Srinivasula, S., et al. 2000. J. Biol. Chem. 275, 36152. Verhagen, A. 2000. Cell 102, 43. |
