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| |  | Cat. No. PK1004 |
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Anti-Janus kinase 1 | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids surrounding Tyr1022/1023 of human JAK1, coupled to KLH | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: CTLL-2 cells, BaF3 cells, and prostate carcinoma | | Comments: Recognizes the ~130 kDa JAK1 protein in CTLL-2 and BaF3 cells. | | Ref.: Leonard, W.J. and O'Shea, J.J. 1998. Annu. Rev. Immunol. 16, 293. Zhu, T., et al. 1998. J. Biol. Chem. 273, 10682. Darnell, J.E. 1997. Science 277, 1630. Argetsinger, L.S., et al. 1995. J. Biol. Chem. 270, 14685. VanderKuur, J., et al. 1995. J. Biol. Chem. 270, 7587. |
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Detection of mouse JAK1 by immunoblotting. Samples: Whole cell lysate from CTLL-2 and BaF3 cells. Primary antibody: Anti-JAK1 Rabbit pAb (Cat. No. PK1004) (1:1000). Detection: chemiluminescence.
Detection of human JAK1 by staining paraffin sections. Sample: Prostate carcinoma. Primary antibody: Anti-JAK1 Rabbit pAb (Cat. No. PK1004) (1:100). Detection: DAB.
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