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PhosphoDetect™ Phospho-specific Antibodies
  PhosphoDetect™ Anti-c-Abl (pThr735) Rabbit pAb
Cat. No. PK1006  
All Categories » Calbiochem » Antibodies » Primary Antibodies » Kinases » Tyrosine

PhosphoDetect™ Anti-Abl1 (pThr735) Rabbit pAb
Anti-Abl1 Phospho-Specific (Thr735) Rabbit pAb
PhosphoDetect™ Anti-p150 (pThr735) Rabbit pAb
Anti-p150, Phospho-Specific (Thr735) Rabbit pAb

Host: Rabbit
Isotype: IgG
Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Thr735 phosphorylation site of human c-Abl, coupled to KLH
Form: Liquid
Formulation: In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preservative: None
Positive Control: Sorbitol-treated HeLa cells
Comments: Recognizes the ~145 kDa c-Abl and the ~190 kDa and ~210 kDa forms of Bcr-Abl phosphorylated at Thr735 in sorbitol-treated HeLa cells.
Ref.: Brasher, B.B. and Van Etten, R.A. 2000. J. Biol. Chem. 275, 35631. Danial, N.N. and Rothman, P. 2000. Oncogene 19, 2523. Pluk, H., et al. 2002. Cell 108, 247. Shaul, Y. 2000. Cell Death Differ. 7, 10.; Wang, J.Y. 2000. Oncogene 19, 5643. Van Etten, R.A. 1999. Trends Cell. Biol. 9, 179.

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CloneSpecies ReactivityApplication
 humanIB 
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Detection of human c-Abl, phosphorylated on Thr735, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with sorbitol (0.5 M, 30 min) or left untreated. Primary antibody: PhosphoDetect™ Anti-c-Abl (pThr735) Rabbit pAb (Cat. No. PK1006) (1:1000) (upper panel) or total c-Abl antibody (lower panel). Detection: chemiluminescence.

Related Literature:

Biologics 32.3

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PK1006: PhosphoDetect™ Anti-c-Abl (pThr735) Rabbit pAb - English
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Related Categories:
All Categories » Calbiochem » Antibodies » Primary Antibodies » Kinases » Tyrosine