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| | PhosphoDetect™ Anti-MAPKAPK-2 (pThr222) Rabbit pAb |
|  | Cat. No. PK1007 |
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Anti-MAPKAPK-2, Phospho-Specific | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Thr222 phosphorylation site of human MAP kinase-activated protein kinase 2, coupled to KLH | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: Anisomycin-treated HeLa cells | | Comments: Recognizes the ~47 kDa MAP kinase-activated protein kinase 2 protein phosphorylated at Thr222 in anisomycin-treated HeLa cells. | | Ref.: Ben-Levy, R., et al. 1995. EMBO J. 14, 5920. Rouse, J., et al. 1994. Cell 78, 1027. |
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Detection of human MAPKAPK-2, phosphorylated on Thr222, by immunoblotting. Samples: Whole cell lysate from HeLa cells left untreated (0 min) or treated with anisomycin for 10, 30, and 60 min. Primary antibody: PhosphoDetect™ Anti-MAPKAPK-2 (pThr222) Rabbit pAb (Cat. No. PK1007) (1:1000) (top panel) and Anti-MAPKAPK-2 (total) (bottom panel). Detection: chemiluminescence.
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