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PhosphoDetect™ Phospho-specific Antibodies
   
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  PhosphoDetect™ Anti-c-Kit (pTyr719) Rabbit pAb
Cat. No. PK1011  
All Categories » Calbiochem » Antibodies » Primary Antibodies » Kinases » Tyrosine

Anti-c-Kit, Phospho-Specific (Tyr719)

Host: Rabbit
Isotype: IgG
Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr719 phosphorylation site of mouse c-Kit, coupled to KLH
Form: Liquid
Formulation: In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preservative: None
Positive Control: Stem cell factor-treated MO7e cells
Negative Control: Untreated MO7e cells
Comments: Recognizes the ~145 kDa c-Kit protein phosphorylated at Tyr719 in stem cell factor-treated MO7e cells. Also recognizes tyrosine-phosphorylated PDGF receptor.
Ref.: Blume-Jensen, P. et al. 2000. Nat. Genet. 24, 157. Hirota, S., et al. 1998. Science 279, 577. Gommerman, J.L., et al. 1997. J. Biol. Chem. 272, 30519. Sattler, M., et al. 1997. J. Biol. Chem. 272, 10248. Martin, F.H., et al. 1990. Cell 63, 203. Nocka, K., et al. 1990. EMBO J. 9, 1805. Yarden, Y., et al. 1987. EMBO J. 6, 3341.
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EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

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50 µl*$228.00

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Cat. No.Disclaimers
PK1011-50UL
Stock StatusPlease contact our Customer Service Department for delivery time.

CloneSpecies ReactivityApplication
 human, mouseIB 
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Detection of human c-Kit, phosphorylated on Tyr719, by immunoblotting. Samples: Whole cell lysate from MO7e cells left untreated (-) or treated with SCF (50 ng/ml for 2 min; +). Primary antibodies: PhosphoDetect™ Anti-c-Kit (pTyr719) Rabbit pAb (upper panel) (Cat. No. PK1011) (1:1000) and Anti-c-Kit (total) (lower panel). Detection: chemiluminescence.

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PK1011: PhosphoDetect™ Anti-c-Kit (pTyr719) Rabbit pAb - English
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All Categories » Calbiochem » Antibodies » Primary Antibodies » Kinases » Tyrosine
 
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are brands of EMD Chemicals Inc., an Affiliate of Merck KGaA, Darmstadt, Germany.