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| | PhosphoDetect™ Anti-p70S6K(pThr389) Rabbit pAb |
|  | Cat. No. PK1015 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Thr389 phosphorylation site of human p70S6K | | Form: Liquid | | Formulation: In Tris citrate/phosphate buffer, pH 7-8. | | Preservative: ≤0.1% sodium azide | | Positive Control: MCF7 cells | | Comments: Recognizes the ~60 kDa p70S6 kinase protein phosphorylated at Tyr389 in MCF7 cells. | | Ref.: Ali, S.M. and Sabatini, D.M. 2005. J. Biol. Chem. 280, 19445. Manning, B.D. 2004. J. Cell. Biol. 167, 399. Lehman, J.A., et al. 2003. J. Biol. Chem. 278, 28130. Pearson, R.B., et al. 1995. EMBO J. 14, 5279. |
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Detection of human p70S6 kinase, phosphorylated on Thr389, by immunoblotting. Samples: Whole cell lysate (50 µg) from MCF7 cells left untreated (lane 2,4) or treated with 100 nM rapamycin for 15 min (lane 3,5); lane 4 and 5: lysates treated with lambda phosphatase. Primary antibody: PhosphoDetect™ Anti-p70S6K (pThr389) Rabbit pAb (Cat. No. PK1015) (1 µg/ml). Detection: chemiluminescence.
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