Detection of human Lck by flow cytometry. Samples: Jurkat cells left untreated (blue line) or treated with anti-CD3 (green line). Primary antibody: Anti-Lck Rabbit pAb (Cat. No. PK1105) (1:100) or irrelevant control antibody (red line). Detection: fluorescence.
Detection of human Lck by immunoblotting. Samples: Whole cell lysate from serum-starved (16 h) Jurkat cells treated with calf intestinal alkaline phosphatase (CIP), left untreated, or treated with H2O2 (2 mM). Primary antibody: Anti-Lck Rabbit pAb (Cat. No. PK1105) (1:1000). Detection: chemiluminescence.
| 
