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| | PhosphoDetect™ Anti-Dynamin (pSer774) Sheep pAb |
|  | Cat. No. PS1000 |
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| Host: Sheep | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser774 phosphorylation site of human dynamin | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Comments: Recognizes the ~100 kDa dynamin protein, phosphorylated at Ser774 in rat brain synaptosomes. | | Ref.: Tan, T.C., et al. 2003 Nat. Cell Biol. 8, 701. Graham, M.E., et al. 2002 Proc. Natl. Acad. Sci. USA 99, 7124. Tsuboi, T., et al. 2002 J. Biol. Chem. 277, 15957. Cousin, M.A., et al. 2001. J. Neurochem. 76, 105. |
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Detection of rat dynamin, phosphorylated at Ser774, by immunoblotting. Sample: Whole tissue extract from rat brain synaptosomes (10 µg). Primary antibody: Anti-Dynamin, Phospho-Specific (Ser774) (Cat. No. PS1000) (1:1000) pre-incubated without blocking peptide (control lane, left lane) or with immunogen phosphopeptide (peptide block, right lane). Detection: chemiluminescence.
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