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| | PhosphoDetect™ Anti-Synaptotagmin (pSer309) Rabbit pAb |
|  | Cat. No. PS1002 |
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Anti-Syt I, Phospho-Specific (Ser309)
Anti-Syt II, Phospho-Specific (Ser309)
Anti-Syt III, Phospho-Specific (Ser309) | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser309 phosphorylation site of human synaptotagmin | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: Rat brain | | Comments: Recognizes the ~60-62 kDa synaptotagmin protein phosphorylated at Ser309, in rat brain. | | Ref.: Poskanzer, K.E., et al. 2003. Nature (London) 426, 559. Wang, C.T., et al. 2003. Nature (London) 424, 943. Fernandez-Chacon, R., et al. 2001. Nature (London) 410, 41. Hilfiker, S., et al. 1998. J. Neurochem. 73, 921. |
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Detection of rat synaptotagmin, phosphorylated at Ser309, by immunoblotting. Sample: Whole tissue extract from rat brain (10 µg). Primary antibody: PhosphoDetect™ Anti-Synaptotagmin (pSer309) Rabbit pAb (1:1000) pre-incubated without blocking peptide (control, left lane) or with immunogen phosphopeptide (block, right lane). Detection: chemiluminescence.
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