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| | PhosphoDetect™ Anti-IFNAR1 (pSer535,539) Rabbit pAb |
|  | Cat. No. PS1003 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser535,539 phosphorylation sites of human IFNAR1 | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: IFNAR1 transfected HEK293 cells | | Comments: Recognizes the ~130 kDa IFNAR1 protein phosphorylated at Ser535,539. | | Ref.: Suresh Kumar, K.G., et al. 2003. EMBO J. 22, 5480. Kirkwood, J., et al. 2002. Semin. Oncol. 29, 18. Biron, C.A., et al. 2001. Immunity 14, 661. Stark, G.R., et al. 1998. Ann. Rev. Biochem. 67, 227. Uze, G., et al. 1990. Cell 60, 225. |
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EMD Chemicals Inc. GBP list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | bovine, human, mouse, rat | DB, IB
See Key |
Detection of human IFNAR1, phosphorylated at Ser535/539, by immunoblotting. Samples: Whole cell lysate from HEK293 cells mock transfected (lane 1), transfected with wild-type IFNAR1 (lane 2), or transfected with S535A/S539A mutant IFNAR1 (lane 3). Primary antibody: PhosphoDetect™ Anti-IFNAR1 (pSer535,539) Rabbit pAb (Cat. No. PS1003) (1:1000) pre-incubated without peptide (control, left panel), with immunogen phosphopeptide (phos-peptide, middle panel), or non-phosphopeptide corresponding to the phosphopeptide immunogen (dephos-peptide, right panel). Detection: chemiluminescence.
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