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| | PhosphoDetect™ Anti-Synaptotagmin (pThr202) Rabbit pAb |
|  | Cat. No. PS1006 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Thr202 phosphorylation site of human synaptotagmin | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: Rat brain extract | | Comments: Recognizes the ~60-62 kDa synaptotagmin protein phosphorylated on Thr202 in rat brain tissue. | | Ref.: Poskanzer, K.E., et al. 2003. Nature 426, 559. Wang, C.T., et al. 2003. Nature 424, 943. Fernandez-Chacon, R., et al. 2001. Nature 410, 41. Hilfiker, S., et al. 1998. J. Neurochem. 73, 921. |
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Detection of rat synaptotagmin, phosphorylated at Thr202, by immunoblotting. Sample: Whole tissue extract (10 µg) from rat brain. Primary antibody: PhosphoDetect™ Anti-Synaptotagmin (pThr202) Rabbit pAb (Cat. No. PS1006) (1:1000) pre-incubated without peptide (control, left lane) or phosphopeptide immunogen (block, right lane). Detection: chemiluminescence.
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