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| | PhosphoDetect™ Anti-c-Met (pTyr1230/1234/1235) Rabbit pAb |
|  | Cat. No. PS1007 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1230/1234/1235 phosphorylation sites of human c-Met in HGF-treated, c-Met-transfected HEK293 cells | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3 (+/- 0.1). | | Preservative: ≤0.1% sodium azide | | Positive Control: 293T human kidney cells transfected with human c-Met and stimulated with HGF, A431 cells stimulated with HGF, or mouse myeloma (SP-1) cells stimulated with HGF | | Comments: Recognizes the ~140-160 kDa c-Met protein phosphorylated at Tyr1230/1234/1235. This antibody does not distinguish between dually (Tyr1234/1235) and triply (Tyr1230/1234/1235) phosphorylated forms of c-Met. | | Ref.: Palka, H.L., et al. 2003. J. Biol. Chem. 278, 5278. Vadnais, J., et al. 2002. J. Biol. Chem. 277, 48342. Crostella, L., et al. 2001. Oncogene 20, 3735. Fan, S., et al. 2001. Mol. Cell. Biol. 21, 4968. Parr, C., et al. 2001. Biochem. Biophys. Res. Commun. 285, 1330. Follenzi, A., et al. 2000. Oncogene 19, 3041. Furge, K.A., et al. 2000. Oncogene 19, 5582. Wei, L., et al. 1995. J. Biol. Chem 270, 8122. Guiton, M., et al. 1994. J. Biol. Chem 269, 30370. |
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* Additional Product Information
Detection of human c-Met, phosphorylated at Tyr1230/1234/1235, by immunoblotting. Samples: Whole cell lysates from 293T cells left unstimulated (lane 1) or HGF-stimulated (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-c-Met (pTyr1230/1234/1235) Rabbit pAb (Cat. No. PS1007) (1:1000) preincubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
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