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PhosphoDetect™ Phospho-specific Antibodies
  PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb
Cat. No. PS1008  
All Categories » Calbiochem » Antibodies » Primary Antibodies » Kinases » Tyrosine

Anti-FADK1, Phospho-Specific (Tyr861)
FAK 1
Anti-pp125FAK, Phospho-Specific (Tyr861)
Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr861)
Anti-PTK2, Phospho-Specific (Tyr861)

Host: Rabbit
Isotype: IgG
Immunogen: a synthetic phosphopeptide surrounding the Tyr861 phosphorylation site of human FAK
Form: Liquid
Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
Preservative: ≤0.1 sodium azide
Positive Control: Chicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
Comments: Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861 in primary chick embryo fibroblasts transfected with human FAK.
Ref.: Eliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149. Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487. Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277. Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735. Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203. Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631. Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295. Nakamura, K., et al. 2001. Oncogene 20, 2626. Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902. Slack, J.K., et al. 2001. Oncogene 20, 1152. Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249. Vial, D., et al. 2000. J. Biol. Chem. 275, 28269. Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.

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100 µlYN/A

CloneSpecies ReactivityApplication
 chicken, human, mouseIB, IH 
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Detection of FAK, phosphorylated at Tyr861, by immunoblotting. Samples: Whole cell lysates from chicken embryo fibroblasts (CEF) expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb (Cat. No. PS1008) (1:1000) pre-incubated with phosphopeptide immunogen (lane 1), a generic phosphotyrosine-containing peptide (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), or without peptide (lane 4). Detection: chemiluminescence.

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PS1008: PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb - English
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Related Categories:
All Categories » Calbiochem » Antibodies » Primary Antibodies » Kinases » Tyrosine