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| | PhosphoDetect™ Anti-IR/IGF1R (pTyr1158/1162/1163) Rabbit pAb |
|  | Cat. No. PS1009 |
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IGF-1
SM-C/IGF-1
Somatomedin-C, Sulfation Factor | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1158/1162/1163 phosphorylation sites of human IR/IGF1R | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50 % glycerol, pH 7.3 (± 0.1). | | Preservative: ≤0.1% sodium azide | | Positive Control: CHO-T cells transfected with human insulin receptor, stimulated with insulin or 3T3-L1 adipocytes stimulated with insulin | | Comments: Recognizes the ~100 kDa IR/IGF1R protein phosphorylated at Tyr1158/1162/1163. | | Ref.: Pender, C., et al. 2002. J. Biol. Chem. 277, 43565. Bevan, P., et al. 2001. J. Cell. Sci. 114, 1429. Skorey, K.I., et al. 2001. Anal. Biochem. 291, 269. Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103. Playford, M.P., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 12103. Ebina, Y., et al. 1985. Cell 40, 747. Ullrich, A., et al. 1985. Nature 313, 756. |
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Detection of human IR, phosphorylated at Tyr1158/1162/1163, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with human insulin receptor and left unstimulated (lane 1) or stimulated with insulin (lanes 2-8). Primary antibody: PhosphoDetect™ Anti-IR/IGF1R (pTyr1158/1162/1163) Rabbit pAb (Cat. No. PS1009) (1:1000) pre-incubated without peptide (lane 1-2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), phosphopeptides corresponding to other IR/IGF1R sites (lanes 5-7), or phosphopeptide immunogen (lane 8). Detection: chemiluminescence.
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