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| | PhosphoDetect™ Anti-IRS-1 (pTyr612) Rabbit pAb |
|  | Cat. No. PS1010 |
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Chico protein dIRS
Anti-Insulin Receptor Substrate-1, Phospho-Specific (Tyr612) | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr612 phosphorylation site of human IRS-1 | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA (IgG, protease free) as a carrrier, pH 7.3. | | Preservative: ≤0.1% sodium azide | | Positive Control: CHO cells expressing human insulin receptor (CHO-T), subsequently transfected with human IRS-1, stimulated with insulin | | Comments: Recognizes the ~165-180 kDa IRS-1 protein phosphorylated at Tyr612. | | Ref.: Aguirre, V., et al. 2002. J. Biol. Chem. 277, 1531. Hers, I., et al. 2002. Biochem. J. 368, 875. Shao, J., et al. 2002. Diabetes 51, 19. Esposito, D.L., et al. 2001. Endocrinology 142, 2833. Fujioka, T., et al. 2001. Eur. J. Biochem. 268, 4158. Shaw, L.M., et al. 2001. Cell. Biol. 21, 5082. Ravichandran, L.V., et al. 2001. J. Biol. Chem. 276, 3543. Reiss, K., et al. 2001. Oncogene 20, 490. Li, J., et al. 1999. J. Biol. Chem. 274, 9351. |
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Detection of human IRS-1, phosphorylated at Tyr612, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with human IRS-1, left untreated (lane 1) or treated with insulin (50 nM for 5 min) (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-IRS1 (pTyr612) Rabbit pAb (Cat. No. PS1010) (0.5 µg/ml) pre-incubated without peptide (lanes 1,2), with a generic phosphotyrosine-containing peptide (lane 3), non-phosphopeptide corresponding to the immunogen (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
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