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| | PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb |
|  | Cat. No. PS1013 |
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Anti-VEGF Receptor 2, Phospho-specific (pTyr1054/1059)
Anti-KDR/Flk-1, Phospho-Specific (Tyr1054/1059) | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1054/1059 phosphorylation sites of mouse and human VEGFR-2 | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (± 0.1). | | Preservative: ≤0.1% sodium azide | | Positive Control: Porcine aortic endothelial (PAE) cells transfected with a CSF-1/mouse VEGFR2 chimeric receptor | | Comments: Recognizes the ~200 kDa VEGFR-2 protein phosphorylated at Tyr1054/1059 in porcine pancreatic cells transfected with a CSF-1/mouse VEGFR-2 chimeric receptor. | | Ref.: Meyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081. Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714. Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201. Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401. |
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* Additional Product Information
Detection of mouse VEGFR2, phosphorylated at Tyr1054/1059, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with CSF-1/mouse VEGFR2 chimeric receptor left unstimulated (lanes 1,2) or stimulated with CSF-1 (lanes 3-8). Primary antibodies: PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb (Cat. No. PS1013) (0.5 µg/ml) pre-incubated without peptide (lanes 2,4,5), with non-phosphopeptide corresponding to the immunogen (lane 6), a generic phosphotyrosine-containing peptide (lane 7), or the phosphopeptide immunogen (lane 8) or pan anti-VEGFR2 antibody (lanes 1,3). Detection: chemiluminescence.
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