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| | PhosphoDetect™ Anti-GSK-3b, (pSer9) Rabbit pAb |
|  | Cat. No. PS1018 |
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PhosphoDetect™ Anti-Glycogen Synthase Kinase-3b (pSer9)
PhosphoDetect™ Anti-Tau Protein Kinase 1 (pSer9)
PhosphoDetect™ Anti-TPK1 (pSer9) | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser9 phosphorylation site of human GSK-3b | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3 (±0.1). | | Preservative: ≤0.1% sodium azide | | Positive Control: Serum-starved 3T3-L1 cells treated with 100 nM insulin or 100 ng/ml IGF-1 | | Comments: Recognizes the ~47 kDa GSK-3b protein phosphorylated on Ser9 in insulin or IGF-1 treated, serum-starved 3T3-L1 cells. | | Ref.: Etienne-Manneville, S., et al. 2003. Nature 421, 753. Eickholt, B.J., et al. 2002. J. Cell Biol. 157, 211. Bhat, R. V., et al. 2000. Proc. Natl. Acad. Sci. 97, 11074. Ding, V.W., et al. 2000. J. Biol. Chem. 275, 32475. Hetman, M., et al. 2002. J. Biol. Chem. 277, 49577. Kitaura, J., et al. 2000. J. Exp. Med. 192, 729. Summers, S.A., et al. 1999. J. Biol. Chem. 274, 17934. Summers, S.A., et al. 1999. J. Biol. Chem. 274, 23858. Weihl, C.C., et al. 1999. J. Neurosci. 19, 5360. |
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Detection of human GSK-3b, phosphorylated at Ser9, by immunoblotting. Samples: Control lysate (lane 1) or control lysated spiked with recombinant GSK-3b (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3b (pSer9) Rabbit pAb (Cat. No. PS1018) (1:1000) preincubated without peptide (lanes 1,5) or with phosphopeptide immunogen (lane 2), non-phosphopeptide corresponding to the immunogen (lane 3), or a generic phosphoserine-containing peptide (lane 4). Detection: chemiluminescence.
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