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PhosphoDetect™ Phospho-specific Antibodies
  PhosphoDetect™ Anti-mTOR (pSer2448) Rabbit pAb
Cat. No. PS1020  
All Categories » Calbiochem » Antibodies » Primary Antibodies » Kinases » Akt/PKB

Anti-FK506 binding protein 12-rapamycin associated protein 1
Anti-FRAP1
Anti-FRAP2
Anti-RAFT1
Anti-RAPT1

Host: Rabbit
Isotype: IgG
Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser2448 phosphorylation site of human TOR
Form: Liquid
Formulation: In Tris citrate/phosphate buffer, pH 7.0-8.0.
Preservative: ≤0.1% sodium azide
Positive Control: EGF-treated HEK293 cells
Comments: Recognizes the ~290 kDa mTOR protein phosphorylated at Ser2448 in EGF-treated HEK293 cells.
Ref.: Kimura, N., et al. 2003. Genes Cells 8, 65. Sekulic, A., et al. 2000. Cancer Res. 60, 3504. Alarcon, C.M., et al. 1996. Genes Dev. 10, 279. Freeman, K., et al. 1996. Gene 172, 143. Cardenas, M.E., et al. 1995. EMBO J. 14, 5892. Lorenz, M.C., et al. 1995. J. Biol. Chem. 270 27531. Sabatini, D.M., et al. 1995. J. Biol. Chem. 270, 20875. Zheng, X.F., et al. 1995. Cell 82, 121. Brown, E.J., et al. 1994. Nature 369, 756. Sabatini, D.M., et al. 1994. Cell 78, 35. Stan, R., et al. 1994. J. Biol. Chem. 269, 32027. Kunz, J., et al. 1993. Cell 73, 585. Heitman, J., et al. 1991. Science 25, 905.

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50 µgY$216.00


CloneSpecies ReactivityApplication
 humanIB 
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Detection of human TOR, phosphorylated at Ser2448, by immunoblotting. Samples: Whole cell lysate (50 µg) from EGF-treated HEK293 cells incubated without lambda phosphatase (lanes 1,2) or with lambda phosphatase (Cat. No. 539514) (lane 3). Primary antibody: Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2) (Cat. No. OP97) (3 µg/ml) (lane 1) and PhosphoDetect™ Anti-mTOR (pSer2448) Rabbit pAb (Cat. No. PS1020) (3 µg/ml) (lanes 2,3). Detection: chemiluminescence.

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Material Safety Data Sheets:
PS1020: PhosphoDetect™ Anti-mTOR (pSer2448) Rabbit pAb - English
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Related Categories:
All Categories » Calbiochem » Antibodies » Primary Antibodies » Kinases » Akt/PKB