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| | PhosphoDetect™ Anti-MARCKS (pSer152/156) Rabbit pAb |
|  | Cat. No. PS1022 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids surrounding the Ser152/156 phosphorylation site of rat MARCKS, coupled to KLH | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM sodium HEPES, 100 µ g/ml BSA, 50 % glycerol, pH 7.5. | | Preservative: None | | Positive Control: NIH/3T3 or C6 cells, serum-starved and treated with TPA | | Comments: Recognizes the ~76 kD MARCKS protein phosphorylated at Ser152 and Ser156 in serum-starved and TPA-treated NIH/3T3 or C6 cells. | | Ref.: Ramsden, J.J., et al. 2000. Int. J. Biochem. Cell Biol. 32, 475. Heemskerk, F.M., et al. 1993. Biochem. Biophys. Res. Commun. 190, 236. Hartwig, J.H., et al. 1992. Nature 356, 618. Thelen, M., et al. 1991. Nature 351, 320. Graff, J.M., et al. 1989. J. Biol. Chem. 264, 21818. |
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Detection of human MARCKS, phosphorylated at Ser152/156, by immunoblotting. Samples: whole cell lysates from NIH/3T3 and C6 cells serum-starved for 16 h and left untreated (-) or treated with TPA (+, 0.2 µM for 30 min). Primary antibody: PhosphoDetect™ Anti-MARCKS (pSer152/156) Rabbit pAb (Cat. No. PS1022) (1:1000). Detection: chemiluminescence.
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