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Cell Adhesion

Kit Resource
  Galectin-3 ELISA Kit
Cat. No. QIA112  
All Categories » Calbiochem » Kits and Tools » Cell Adhesion and Migration Assays

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric
Species Reactivity: human
Sensitivity: 120 pg/ml
Assay Range: 0.16 - 10 ng/ml
Assay Time: 4 h
Sample Type: Cell culture supernatants, plasma, serum, or other biological fluids
Positive Control: Galectin-3
Kit Contents: Coated Microplate, Anti-Galectin-3 (Rabbit), Anti-Rabbit-HRP, Galectin-3 Standard (2 vials), Assay Buffer Concentrate, Wash Buffer Concentrate, Sample Diluent, Substrate Solution I, Substrate Solution II, Stop Solution, Blue-Dye, Red-Dye, Green-Dye, Plate Covers, and a user protocol.
Comments: Designed for the quantitative detection of human Galectin-3.
Ref.: Liu, F.T. 2000. In Lectins and Pathology. (Caron, M. and Seve, D. eds.) Harwood Academic Publishers, Amsterdam, The Netherlands. Sano, H., et al. 2000. J. Immunol. 165, 2156. Cooper, D.N., and Barondes, S.H. 1999. Glycobiology 9, 979. Kim, H.R., et al. 1999. Cancer Res. 59, 4148. Nachtigal, M., et al. 1998. Am. J. Pathol. 152, 1199. Bresalier, R.S., et al. 1997. Cancer 80, 776. Fernandez, P.L., et al. 1997. J. Pathol. 181, 80. Castronovo, V., et al. 1996. J. Pathol. 179, 43. Hsu, D.K., et al. 1996. Am. J. Pathol. 148, 1661. van den Brüle, F.A., et al. 1996. Hum. Pathol. 27, 1185. Yang, R.Y., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6737. Dagher, S.F., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 1213. Liu, F.T., et al. 1995. Am. J. Pathol. 147, 1016. Sato, S., and Hughes, R.C. 1994. J. Biol. Chem. 269, 4424. van den Brüle, F.A., et al. 1994. Eur. J. Cancer 30A, 1096. Lindstedt, R., et al. 1993. J. Biol. Chem. 268, 11750. Liu, F.T. 1993. Immunol. Today 14, 486. Lotz, M.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3466. Nangia-Makker, P., et al. 1993. Cancer Res. 53, 5033. Agrwal, N., et al. 1989. J. Biol. Chem. 264, 17236. Laing, J.G. and Wang, J.L. 1988. Biochemistry 27, 5329. Flotte, T.J., et al. 1983. Am. J. Pathol. 111, 112.

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Inter-Assay Reproducibility.


Intra-Assay Reproducibility.


Parallelism.


Standard Curve. Recombinant, soluble, human galectin-3 was diluted in serial two-fold steps in Sample Diluent. The results represent the mean of three parallel titrations.


Typical Data Using the Galectin-3 Standard.

Material Safety Data Sheets:
QIA112: Galectin-3 ELISA Kit - English
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