| Format: Flow cytometry or fluorescence microscopy | | Form: 100 Tests | | Detection Method: Fluorescence | | Species Reactivity: a broad range of species | | Assay Time: 1.5 h | | Sample Type: Intact cells | | Kit Contents: Labeled Caspase-8 Inhibitor (Red-IETD-FMK), Wash Buffer, Unlabeled Caspase Inhibitor (Z-VAD-FMK), and a user protocol. | | Comments: A fast, convenient, and sensitive method to measure caspase-8 in living cells. The fluorescent marker, Red-IETD-FMK, is a labeled, cell-permeable, non-toxic inhibitor that binds irreversibly to activated caspase-8 in living cells. Caspase-8 can be detected by the measurement of fluorescence intensity in a fluorescent microplate reader, flow cytometer, or by fluorescence microscopy. |
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