Format: Flow cytometry or fluorescence microscopy

Form: 100 Tests

Detection Method: Fluorescence

Species Reactivity: a broad range of species

Assay Time: 1.5 h

Sample Type: Intact cells

Kit Contents: Labeled Caspase-9 Inhibitor (Red-LEHD-FMK), Wash Buffer, Unlabeled Caspase Inhibitor (Z-VAD-FMK), and a user protocol.

Comments: A fast, convenient, and sensitive method to measure caspase-9 in living cells. The fluorescent marker, Red-LEHD-FMK, is a labeled, cell-permeable, non-toxic inhibitor that binds irreversibly to activated caspase-9 in living cells. Caspase-9 can be detected by the measurement of fluorescence intensity in a fluorescent microplate reader, flow cytometer, or by fluorescence microscopy.