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 | Multiple Storage Requirements |  | Multiple conditions |  | Hazardous |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | Calpain Activity Assay Kit, Fluorogenic | |  | Cat. No. QIA120 | |
| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Fluorometric | | Assay Time: 1.5 h | | Sample Type: Cell lysates, plasma, serum | | Positive Control: Calpain 1 | | Kit Contents: Calibration Standard, Positive Control Calpain 1, Substrate, Activation Buffer, Inhibition Buffer, Reduction Agent, Assay Buffer, Cell Lysis Buffer, 96-Well Plate, Plate Sealer, and a user protocol. | | Comments: Calpains are Ca2+-dependent proteinases, which are involved in normal cellular processes including cell proliferation, apoptosis, differentiation, and cell migration. Requires a fluorimeter or microplate reader capable of measuring fluorescence at wavelengths of Excitation max: ~360 nm and Emission max: ~460 nm. | | Ref.: Dainese, E., et al. 2002. J. Biol. Chem. 277, 40296. Glading, A., et al. 2000. J. Biol. Chem. 275, 23908. Ishikara, I., et al. 2000. Neurosci. Lett. 279, 97. Nakagawa, T. and Yuan, J. 2000. J. Cell Biol. 150, 887. Pariat, M., et al. 2000. Biochem. J. 345, 129. Pink, J.J., et al. 2000.Exp. Cell Res. 255, 144. Wang, K.K. 2000. Trends Neurosci. 23, 20. Reddy, R.K., et al. 1999. J. Biol. Chem. 274, 28476. Leist, M., et al. 1998. Mol. Pharmacol. 54, 789. Melloni, E., et al. 1998. J. Biol. Chem. 273, 12827. Villa, P.G., et al. 1998. J. Cell Sci. 111, 713. Wood, D.E., et al. 1998. Oncogene 17, 1069. Kubbutat, M.H. and Vousden, K.H. 1997. Mol. Cell Biol. 17, 460. Molinari, M. and Carafoli, E. 1997. J. Membr. Biol. 156, 1. Sorimachi, H., et al. 1997. Biochem. J. 328, 721. Squier, M.K. and Cohen, J.J. 1997. J. Immunol. 158, 3690. Aoki, K., et al. 1986. FEBS Lett. 205, 313. Ohno, S., et al. 1986. Nucleic Acid Res. 14, 5559. | |
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* Additional Product Information
 Typical Standard Curve. Typical standard curve generated by using dilutions of the AMC Standard as indicated in the Detailed Protocol.
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 | - Afsaneh Abdolzade-Bavil, et al. (2004) Convenient and versatile subcellular extraction procedure, that facilitates classical protein expression profiling and functional protein analysis. Proteomics 4, 1397-1405.
- Ashish K. Sharma and Baerbel Rohrer. (2004) Calcium-induced Calpain Mediates Apoptosis via Caspase-3 in a Mouse Photoreceptor Cell Line. Journal of Biological Chemistry 279, 35564-35572.
- Gloria Bonuccelli, et al. (2003) Proteasome inhibitor (MG-132) treatment of mdx mice rescues the expression and membrane localization of dystrophin and dystrophin-associated proteins. American Journal of Pathology 163, 1663-1675.
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